Single-stranded DNA (ssDNA) is a molecule composed of a single strand of nucleotides, which are the building blocks of DNA. Double-stranded DNA (dsDNA), which consists of two complementary strands that are connected by hydrogen bonds. In contrast, ssDNA exists as a lone strand without a complementary partner. ssDNA is involved in various biological processes, including DNA replication, transcription, and recombination. It is also used in molecular biology techniques such as DNA sequencing, DNA hybridisation, and polymerase chain reaction (PCR).
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